Additional Cell Culture Reagents and Supplements
Gibco™ Sodium Bicarbonate 7.5% solution
Commonly used for maintaining the pH of cell culture medium in the presence of 4 to 10% carbon dioxide
Gibco™ 2-Mercaptoethanol
A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals
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Gibco™ HAT Supplement (50X)
HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells
Gibco™ HEPES (Ultra Pure)
A zwitterionic organic chemical buffering agent commonly used in cell culture media
Gibco™ B-27™ Supplement (50X), custom
The custom B-27 Supplement (50X) is a serum-free additive for the growth and expansion of retinal ganglion cells.
Gibco™ Agarose (4%)
An autoclaved, high purity, low melting point gel designed for plaque assays and the purification of baculovirus
Corning™ Poloxamer 188, Powder
Acts as surfactant used in cell culture to protect cells in suspension against possible damage during transfer, freezing and thawing, and stirring.
Gibco™ Autolyzed Yeast Extract (15% solution)
A concentrated extract of soluble amino acids, carbohydrates, vitamins and minerals
Gibco™ Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)
A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells
MilliporeSigma™ Polybrene Infection/Transfection Reagent
A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors.
